Not Effective for Western Blot

June 23, 2022

Genetics
Emory University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Recombinant Anti-Neurogenin 2/NGN2 antibody

Catalog Number:

ab109236

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: NGN2 Antibodies

I performed Western blot on a control sample of human cortical organoids and a NGN2 knockout line, but the NGN2 antibody did not work. I do not recommend buying this for WB.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cortical Organoids

Primary Incubation

1:1000 overnight at 4C

Blocking Agent

5% milk

Secondary Incubation

1:1000 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP at RT for 1 hour

Tertiary Incubation

N/A

Detection

Pierce™ SuperSignal™ West Pico PLUS Chemiluminescent

Results Summary

I stained for actin as my control, which worked for both samples. However, this NGN2 antibody did not work at all for Western blot.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

N/A

The Bad

It didn't work.

The Bottom Line

Don't use for Western Blot.

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