Human IgG Fc Antibody

MIRC
McMaster University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human IgG Fc Recombinant Antibody

Catalog Number:

366904

Clone Number:

QA19A42

I use this antibody as a secondary antibody to detect binding of an Fc-tagged recombinant protein to my CAR-NK cells. The antibody yields good staining with a little background, but not too much.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CAR-NK cells

Primary Incubation

0.1ug recombinant protein

Blocking Agent

Fc block

Secondary Incubation

1:20 @ 4C for 30 min

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

The antibody gives a bright signal that can be distinguished from background. With this being a PE conjugated antibody there is some background as expected, but not too much.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good staining.

The Bad

Some background.

The Bottom Line

Works well.

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