Easy Overarching ErbB Array

MGH
Cancer Research
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

RayBiotech

Product Name:

Human EGFR Phosphorylation Array C1

Catalog Number:

AAH-PER-1-8

I used this EGFR array to test changes in cellular expression across the entire ErbB family RTK's under different drugging conditions. In order to better translate into future western blot analysis, this array was very useful in determining which ErbB was greatest impacted but also which phospho-site.

Experimental Design and Results Summary

Application

Cellular ErbB RTK Expression Changes

Starting Material

KRAS G12C mutant Cancer line H2122

Tips

Quantitative analysis requires assumptions from positive controls. Yields relative quantitative results only, simple to do.

Results Summary

Overall, this array was very helpful in determining the next steps in my project experiments. The assay is simple to perform and easy to understand.

DOI or PMID #

N/A

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Summary

The Good

Easy to use and good signal

The Bad

Expensive

The Bottom Line

Great, would definitely use again. Recommend to anyone needing to distinguish between these RTK family members

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