Excellent Cytokine Array Kit

April 15, 2022

Versiti Blood Research Institute
Translational Glycomics
Postdoctoral Researcher

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Ease-of-Optimization

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Company:

R &D Systems

Product Name:

Proteome Profiler Array - Mouse Cytokine Array Kit Panel A

Catalog Number:

ARY006

I used this cytokine array kit to see differences in the expression of cytokines between MPL+DNM2 double knockout mice and MPL knockout mice. Bone marrow supernatant sample was used for the experiment.

Experimental Design and Results Summary

Application

To see differences in Cytokine expression in mice of different genotype.

Starting Material

Bone marrow plasma supernatent

Tips

Prepare wash buffer dilution in advance. Crystals can form in the concentrate, so make sure to thaw well in advance.

Results Summary

Results showed differences in at least 3 cytokines between the Mpl+Dnm2 double knockout vs the Mpl knockout mice.

DOI or PMID #

N/A

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Summary

The Good

Best thing is the easy understanding of the protocol.

The Bad

Overnight incubation step takes a lot of time away.

The Bottom Line

Excellent kit for seeing differences in cytokine expression in mouse model using bone marrow supernatant as sample.

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