CXCL10 From Primary Brain Endothelial Cells

March 14, 2022

University of Illinois at Chicago
Anatomy and Cell Biology
Graduate Student

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Company:

R&D systems

Product Name:

Mouse CXCL10/IP-10 DuoSet ELISA

Catalog Number:

DY466-05

Secreted CXCL10 was measured by ELISA with supernatant from primary brain endothelial cells treated with IFNg.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Cell culture supernatant

Tips

Requires purchase of additional kit R&D DY008

Results Summary

CXCL10 secretion from primary mouse brain endothelial cells increased 5-fold with IFNg treatment.

DOI or PMID #

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Summary

The Good

All reagents needed are provided in the kit

The Bad

Very time-consuming protocol

The Bottom Line

This ELISA kit provided clear and reproducible results. However, this kit requires another kit - DuoSet Ancillary Reagent Kit 2 DY008 - to use and the assay takes 7-8 hours to run.

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