Anti-HA.11 Antibody from BioLegend

January 27, 2022

Division of Cancer Sciences
University of Manchester
Research Scientist

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Company:

Biolegend

Product Name:

Anti-Ha-11 Epitope Tag

Catalog Number:

901513

Clone Number:

16B12

Info:Supplier Page View Product Specs

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This is an exceptional antibody for both immunoblotting and immunofluorescence assays of Ha-tagged proteins. It works quite well in both HEK293 and U2OS cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

U2OS cells

Primary Incubation

1:4000

Blocking Agent

1% BSA in TBST

Secondary Incubation

1:10000, anti-mouse HRP-conjugated antibody

Tertiary Incubation

N/A

Detection

Chemiluminescence, ECL Western Blotting Substrate

Results Summary

Extremely concentrated antibody which works very well for the detection of Ha-tagged proteins in immunoblotting and immunofluorescence assays with lysates from HEK293 and U2OS cells.

DOI or PMID #

Additional Notes

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Summary

The Good

Very concentrated antibody

The Bad

Nothing bad to say about it

The Bottom Line

Extremely concentrated antibody which works very well for the detection of Ha-tagged proteins in immunoblotting and immunofluorescence assays.

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