Thermo Fisher
AIRE Monoclonal Antibody (5H12), Alexa Fluor 488, eBioscience™ Invitrogen AIRE Monoclonal Antibody (5H12), Alexa Fluor 488
53-5934-82
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This antibody was used for staining the intracellular transcription factor Aire in medullary thymic epithelial cells. We were testing if various genetic perturbations altered the expression of Aire.
Flow Cytometry
Mouse thymus
1:200 dilution for 30 minutes at room temp
2.4G2 Fc Block
N/A
LSRII FACS Analyzer
Has a really clean, clear signal. We were able to see that our treatment did not alter Aire expression at all.
Clean signal, doesn't need a high concentration
On the expensive side
For intracellular Aire staining in thymus, this antibody works perfect.