Good Actin Filaments Staining Dye Phalloidin Red

November 19, 2021

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

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Company:

Sigma-Aldrich

Product Name:

Phalloidin–Tetramethylrhodamine B isothiocyanate

Catalog Number:

P1951

Info: Supplier Page 

I have used this dye (Phalloidin–Tetramethylrhodamine B isothiocyanate) from Sigma-Aldrich for the visualization of the cytoskeleton actin filaments in the stem cells treated with or without the drug.

Experimental Design and Results Summary

Application

Immuno-staining/fluorescence

Starting Material

Primary Human cells

Tips

Carefully prepare the stock solution in methanol

Results Summary

Red color filaments like structures observed in fluorescence microscopy confirming the F-actin filaments.

DOI or PMID #

N/A

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Summary

The Good

Easy to use and very low dye concentration is required fot the assay.

The Bad

Stock preparation requires methanol therefore stocks should not be leave open for long time.

The Bottom Line

This dye is compatible with the red channel of the fluorescence microscope, therefore, fits for most of the staining assay.

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