Cell Signaling Technology
TCF1/TCF7 (C63D9) Rabbit mAb (PE Conjugate)
14456S
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Analysis of different subsets of T cells in the spleen to compare TCF levels.
Flow Cytometry
Mouse splenocytes
1:50 dilution for 30 minutes at room temp
2.4G2 Fc Block
N/A
LSRII FACS Analyzer
Found that most T cell subsets in the spleen express comparable levels of TCF, but higher levels than other non-T cells.
Works well at advertised concentrations
Requires high concentration and somewhat expensive
A little pricy but provides good data.