Reliable Western Blot Antibody pATM

November 17, 2021

Department of Internal Medicine
East Tennessee State University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Phospho-ATM (Ser1981) Rabbit mAb

Catalog Number:

5883S

Clone Number:

D6H9

Info: Supplier Page View Product Specs

Browse Similar Products: PhosphoATM Antibodies

A great antibody with fine bands and a very good separation against total ATM. No cross contamination.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human T cells

Primary Incubation

1:1000 in BSA (overnight)

Blocking Agent

5% Milk

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Western Blot (Chemiluminescence)

Results Summary

Antibody was used to detect pATM levels and was easy to detect. Bands were observed within 20-30 seconds of chemiluminescence exposure.

DOI or PMID #

https://doi.org/10.1128/JVI.01061-20

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to detect

The Bad

use 8% gel. 12% cannot detect

The Bottom Line

Will use it again. Great antibody

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