Very Bright Fluorophore and Good Antibody

August 26, 2021

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cyanine7 anti-mouse CD4 Antibody

Catalog Number:

100528

Clone Number:

Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse draining lymph node

Primary Incubation

1:100 in FACS Buffer at RT for 30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometry

Results Summary

great resolution from negative vs. positive populations.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

consistent results across different batches

The Bad

pricy without academic discount

The Bottom Line

Reliable Antibody for flow analysis. Try 1:100 dilution in FACS buffer first. Titration might be needed for optimized results.

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