Invitrogen
ZBP1 antibody
PA5-20455
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To examine the expression levels of ZBP1 in MEF cells, whole cell lysates were made with the buffer [1% SDS, 10 mmol/L Tris-Cl (pH 7.6), 20 μg/μL aprotinin, 20 μg/μL leupeptin, and 1 mmol/L 4-(2-aminoethyl)benzenosulfonyl fluoride]. The protein concentrations were determined using the Bicinchoninic Acid Protein Assay kit (Pierce, Rockford, IL). Fiften micrograms of protein were separated on SDS-PAGE gels and transferred to polyvinylidene difluoride membranes. After blocking, the membranes were incubated with the appropriately diluted primary antibody (1/1000) at 4°C overnight. After washing with TBST, the membranes were incubated with sheep anti-rabbit diluted HRP-conjugated secondary antibody (1/10000) at room temperature for 1 hour. Proteins were detected with the enhanced chemiluminescence kit (Amersham Pharmacia Biotechnology, Inc., Piscataway, NJ).The results showed that the expression levels of ZBP1 were decreased compared to the control.
Western Blot
mouse embryonic fibroblasts
1/1000 at 4°C overnight
5% milk
Sheep anti-rabbit diluted HRP-conjugated secondary antibody (1/10000) at room temperature for 1 hour
Room temperature for 1 hour,
Enhanced chemiluminescence kit (Amersham Pharmacia Biotechnology, Inc., Piscataway, NJ)
The results showed that the expression levels of ZBP1 were decreased compared to the control.
The antibody works
There are many non-specific bands