Cell Signaling Technology
Phospho-4E-BP1 (Thr37/46)
2855S
236B4
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The mouse tissues were homogenized and proteins were extracted in RIPA buffer. The extracted proteins were measured, and an equal amount of proteins were resolved in 12% SDS-PAGE. High range protein marker was used for this experiment. Resolved proteins were further immunoblotted for the detection of Phospho-4E-BP1 protein using specific antibody obtained from Cell Signaling Technology.
Western Blot
Mouse tissue protein extract
Dilution (1:500) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree
5% skimmed milk made in 1x TBST
Goat anti-Rabbit IgG HRP antibody, dilution (1:5000) in 5% skimmed milk made in 1x TBST, 1hr at RT
N/A
ECL
This antibody had detected a few non-specific epitopes, but the specificity was high for target Phospho-4E-BP1.
Gives a specific band of Phospho-4E-BP1 protein
Works food