The Antibody Stains CD86+ Cells Well

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher

Product Name:

CD86 (B7-2) Monoclonal Antibody (GL1), Super Bright 436, eBioscience™

Catalog Number:

62-0862-82

Clone Number:

GL1

We wanted to examine cell activation in the periphery in the healthy vs diseased state, so we used this antibody to identify CD86+ cells in the blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

single-cell suspension of mouse blood cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Attune NxT cytometer

Results Summary

The antibody stained CD86+ cells in the blood pretty clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies CD86+ cells pretty well

The Bad

None to mention

The Bottom Line

The antibody is good for examining CD86+ expression on immune cells

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