Strong Staining for CD8+

April 23, 2021

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher

Product Name:

CD8a Monoclonal Antibody (53-6.7), PE-eFluor 610, eBioscience™

Catalog Number:

61-0081-82

Clone Number:

53-6.7

We wanted to examine T cells in the healthy vs diseased state, so we used this antibody to identify CD8+ T cells in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Attune NxT cytometer

Results Summary

The antibody stains CD8+ T cells very clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody clearly stains CD8+ T cells

The Bad

None to mention

The Bottom Line

The antibody is great for identifying CD8+ T cells in the spleen

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