Works Great for Measuring DMC1 Foci

Pharmology
University of Kansas Medical Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

anti-DMC1

Catalog Number:

HPA001232-100ul

Clone Number:

N/A

DNA repair proteins are necessary for the maintenance of chromosome integrity and are involved in the elimination of premutagenic lesions from DNA. Dmc1 is specifically involved in meiotic recombination. Dmc1 transcript is detected in both male and female germ cells, indicating that the encoded protein may have a role in mammalian genetic recombination in meiosis.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse male germ cell

Primary Incubation

1:200 dilution, 4 degree overnight

Blocking Agent

Donkey serum

Secondary Incubation

1:500 dilution, room temp for 1hour

Tertiary Incubation

None

Detection

Fluorescence microscopy

Results Summary

DMC1 staining was excellent. DMC1 foci were fully resolved from cell.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and highly specific

The Bad

None

The Bottom Line

Works well! Works well! Highly recommended.

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