Invitrogen
Anti-Mouse CD274 (PD-L1) Antibody
14-5982-82
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Mouse breast cancer cells were analyzed for PD-L1 expression by flow cytometry. PD-L1 is an immune marker found on stromal cells activated by cancer.
Flow Cytometry
Mouse breast cancer cells
0.25 ug, 1 hour on ice
1% BSA in PBS
Anti-mouse FITC secondary, 1:1000
N/A
Flow cytometry analysis some cells were indeed positive for PD-L1 expression. Thus, active immune cells were present in the tumor specimen.
Easy to use and good detection
Optimization needed
The antibody can be used for PD-L1 expression. Expensive but recommended.