CD133 PE-eFluor 610 Antibody is adequate for flow cytometry

January 11, 2021

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

CD133 PE-eFluor 610 Antibody

Catalog Number:

61-1338-42

Clone Number:

N/A

Human cervical cancer cells were sorted for CD133-positive or negative expression by flow cytometry following staining with ALDEFLUOR. CD133 and ALDEFLUOR-positive cells were sorted and analyzed for cancer stem cell characteristics.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human cervical cancer cells

Primary Incubation

1:200, 1 hour on ice

Blocking Agent

ALDEFLUOR Binding Buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

Some cells were found to be positive for CD133 by flow cytometry (and co-expressing ALDEFLUOR). Cells appear to have formed tumorspheres in culture.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Directly conjugated so easy to detect cells and does not need extra washing step

The Bad

Optimization needed

The Bottom Line

The antibody enables detection of cells with cancer stem cell marker CD133 by flow cytometry and is compatible with other popular assays such as ALDEFLUOR.

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