Bcl-6 Antibody Works on Human Cells

Microbiology and Immunology
Columbia
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 594 anti-mouse/human Bcl-6 Antibody

Catalog Number:

648308

Clone Number:

IG191E/A8

Our lab studies development and function of human immune cells. We utilize flow cytometry to identify and study cell population of interest in different tissues of the human body.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human blood

Primary Incubation

1:50, 30min ice

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek Aurora

Results Summary

Good positive stain on human CD4 T cells. Antibody allowed for examination of intracellular expression of this transcription factor in CD4 T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Worked well and is bright.

The Bad

Has some background.

The Bottom Line

great signal but need to watch for high background.

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