Great Western Antibody to Assess p-p38 Activity

December 05, 2020

Immunobiology
Yale University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies (CST)

Product Name:

Phospho-p38 MAPK (Thr180/Tyr182) XP® Rabbit mAb

Catalog Number:

#4511

Clone Number:

(D3F9)

p38 is a canonical kinase involved in signaling to regulate cellular stress. We use this antibody to check activity of p38.

Experimental Design and Results Summary

Applications

Western Blot

Sample

T cells

Primary Incubation

1:10000 in 4% BSA/TBST

Blocking Agent

5 percent milk in TBST

Secondary Incubation

1:5000 anti-Rabbit HRP in 5% milk/TBST for 1hour

Tertiary Incubation

None

Detection

Digital imager using ECL

Results Summary

We are able to see increased p-p38 activity upon stimulation in our conditions.

DOI or PMID #

N/A

Additional Notes

Titrate antibody based on samples.

Image Gallery

Summary

The Good

Very good antibody at high dilution to save money.

The Bad

None

The Bottom Line

Highly recommend when studying p38.

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