Antibody Works Well

Dept Imaging Sciences 4th floor, Lambeth Wing, King's College London, St Thomas' Hospital
King's College London
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Santa Cruz Biotechnology

Product Name:

c-Myc Antibody (9E10) - FITC

Catalog Number:

sc-40 FITC

Clone Number:

sc-40

I used this product to determine the transfection efficiency of my myc-tag-containing construct onto target cells. This antibody gives excellent readings after as little 10mins incubation and has a very low background/non-specific staining

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T-cells

Primary Incubation

1uL/million cells, 15mins, room temperate

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

I have used this product consistently for over 2 years to help determine how efficiently my construct has been transduced into my target cells (human T-cells). In our study we used 9e10-FITC antibody to study CAR T-cells persistence and proliferation. The antibody is efficient and works really well, giving nice separation between positive and negative populations

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very accessible and easy to use. Gives great separation between positive and negative populations for easy analysis

The Bad

None

The Bottom Line

Excellent antibody for flow cytometry analysis

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