Abcam
Anti-Xanthine Oxidase antibody
ab233148
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Human cells were growth-arrested, treated with a specific growth factor; protein extracts were made and resolved in a 10% SDS PAGE. Resolved proteins were western transferred in the nitrocellulose membrane and immunodetected for Xanthine Oxidase protein levels. Selection of antibody was based on the information provided in the flyer.
Western Blot
Human cell protein extract
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk made in 1x TBST
Goat anti-Rabbit IgG HRP, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT
None
Enhanced chemiluminescent detection in X-Ray film
Indicated Xanthine Oxidase antibody from Abcam has detected specific band of Xanthine Oxidase at the correct size. However, bright non-specific signals were also detected. There were a effect of growth factor treatment on Xanthine Oxidase levels in the cells.
none
Gives specific band
Few non-specific bands were detected
Good antibody