Detection of cJun Expression in BV2 Microglia Cells

January 04, 2021

Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

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Company:

Cell Signaling

Product Name:

c-Jun (60A8) Rabbit mAb

Catalog Number:

9165

Clone Number:

N/A

In our experimental project we determined downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigated the effect of LPA on inflammatory transcription factors in microglia cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 microglia cells

Primary Incubation

1:1000 overnight at +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 goat anti-rabbit, 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ChemiDoc Development System

Results Summary

No changes were observed in cJun expression after LPA treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Rather good signal, reproducible results

The Bad

N/A

The Bottom Line

Highly recommended.

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