Cell Signaling
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4668
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Primary murine microglia were treated with LPA in the presence or absence of LPAR5 (TCLPA5) or PKD family (CRT) inhibitors in order to detect changes in the phosphorylation of MAPK family members.
Western Blot
Primary murine microglia
1:500 overnight at +4
5% milk
1:5000 goat anti-rabbit, 2 hours at room temperature
N/A
ChemiDoc System
The LPA induced activation of JNK appeared to be affected after inhibition of LPAR5 and PKD family.
Reliable and efficient results.
In some cases, even though the conditions and the amount of protein loaded are the same, the signal is not so good.
Generally a good antibody for the detection of phospho JNK.