Cell Signaling Technologies
P-PKC alpha and Beta II
9375S
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Hyperoxia leads to development of reactive oxygen species (ROS). These ROS cause oxidative damage to the lung, nucleic acids, and proteins, leading to cellular injury and inflammation. Here we found time dependent increase of P-PKC alpha and Beta IIby chemoattractant.
Western Blot
Cell and mouse tissue lysate samples
1:1000 dilution in 2% skim milk prepared in 1X TBST
2% skimmed milk
1:4000 5% skim milk prepared in 1X TBST
None
ECL
We found time dependent increase of P-PKC by chemoattractant.
N/A
No background and very strong signal
This antibody is very good and the signal is strong.