Abcam
Anti-FOXM1 antibody
ab180710
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I used this antibody to the levels of FOXM1 in mouse (C57BL6) spleen, thymus and lymph nodes.
Western Blot
Mouse primary cells
1:1000 dilution, 18 hours at 4 degrees with gentle shaking
5% non-fat dry milk in Tris buffer containing 0.1% Tween-20
HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking
not applicable
ECL based detection
Cells derived from mouse spleen and thymus showed relatively higher expression of FOXM1 than lymph nodes.
high sensitivity
This antibody is polyclonal and gives some non specific bands in western blot.
Overall, this antibody works well for western blot.