High Quality Rabbit Anti-Olig2 Antibody

September 15, 2020

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Rabbit Anti-Olig2 antibody

Catalog Number:

AB9610

Clone Number:

N/A

Antibody of high quality for researchers studying oligo2+ cells during development.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryo E11.5: Spinal cord

Primary Incubation

1:300, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

1:500, 2h at RT, in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

Not done.

Detection

Confocal microscope.

Results Summary

pMN domain labeled by Olig2 staining. Easy to optimise. Vessel background shown in the uploaded image is due to the secondary antibody used.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Quality.

The Bad

Price.

The Bottom Line

Perfect antibody to detect Olig2+ Cell during brain and spinal cord development.

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