eBioscience
Streptavidin APC Conjugate
17-4317-82
NA
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CXCR3+ plasma cells using anti-CXCR3 biotin.
Flow Cytometry
C57BL/6 spleen
anti-CXCR3 biotin (1/400), anti CD138 BV421 (1/800) 15', 4°
Fc block (2.4G2 clone)
Streptavidin APC (1/400)
Flow cytometer
We could reveal the CXCR3 biot using this SA-APC, but there is some unspecific staining. This can be observed in the control that does not have primary antibody.
It works
There is unspecific staining
There are better streptavidin-APCs on the market.