Thermo Fisher Scientific
Invitrogen Qubit™ 4 Fluorometer
Q33238
Info: Supplier Page
Quantification of DNA samples by Nanodrop spectrophotometer often results in over estimation of the nucleic acids present in the sample. I used Thermo Fisher Scientific's Qubit 4 Fluorometer for quantification of genomic DNA in my sample. It gave estimation of the genomic DNA since it measures specific DNA based on the fluorescence. The libraries prepared based on the Qubit quantification gave expected cluster generation for sequencing.
Quantification of genomic DNA samples for preparation of NGS libraries
Thaw Qubit assay reagent at room temperature for 20 minutes.
Qubit Fluorometer 4 also measures RNA integrity, which is an added feature for RNA quality checking.
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Highly recommended for DNA/RNA quantification for sensitive applications like sequencing.