High Cell Density Cultivation of E. coli

University of Birmingham
School of Biosciences
Postdoctoral Research Fellow

Overall

Performance

Ease-of-Optimization

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Company:

INFORS HT

Product Name:

Minifors 2 Benchtop Fermenter

Catalog Number:

NW145

INFORS HT's Minifors 2 bench top fermenter is a simple lab scale fermenter, which is very easy to set up and run a high cell density cultivation of microbial cells. Being a novice in fermentation area, I used this fermenter, which is very intuitive and easy to set up. Using 4 L scale up, I was successful in growing e. coli cells to a cell density of 100 OD units in 18 hours of growth.

Experimental Design and Results Summary

Application

Lab scale (upto 4 L) high cell density growth or cultivation of microbial cells

Tips

When setting up the fermenter, make sure that exit gas cooler filter is not wet or blocked.

DOI or PMID #

None

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Summary

The Good

Very simple to set up and run. Easy to use. Comes with software to analyse all the fermentation run parameters.

The Bad

None

The Bottom Line

Highly recommended in the Bioprocessing labs and laboratory looking for lab-scale fermentations.

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