Efficient Amplification of DNA Using GoTaq® Green Master Mix

Johns Hopkins University School of Medicine
Department of Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Promega Corporation

Product Name:

GoTaq® Green Master Mix

Catalog Number:

M7121

We use this GoTaq® Green Master Mix for amplification of DNA templates by PCR. I usually perform a pcr using this premixed ready-to-use Master Mix on a liquid culture of E.coli.. I can directly monitor the progress during electrophoresis by checking the dyes (blue and yellow). It takes less than 1 hour to see the results.

Experimental Design and Results Summary

Application

Efficient amplification of DNA templates by PCR.

Starting Material

Bacteria culture

Tips

Perform the experiment according to manufacturer's instructions. This reagent is user friendly.

Results Summary

DNA amplification reaction works well and could be visualized by agarose gel electrophoresis.

Image Gallery

Summary

The Good

Reliable, user friendly

The Bad

None

The Bottom Line

I recommend this because it saves a lot of time, and works well. I didn't recycle the gel product.

Join the discussion