Pre-treated Chip for your NGS Workflow

Osmania University
Department of Biochemistry
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Ion PI Chip kit V3

Catalog Number:

A26770

Next-generation sequencing is a high throughput technique where s huge number of samples can be simultaneously analyzed. The DNA barcode kit can be used to barcode each and every sample in the analysis with a specific barcode sequence. We have used this barcode kit to code three different experimental replicates of the Rat H9C2 cell line for performing the CHIP assay.

Experimental Design and Results Summary

Application

Next Generation Sequencing

Starting Material

DNA/RNA libraries

Tips

The chips should be used before 8 months from the date of manufacturing

Results Summary

A very good loading; more than 80% loading is obtained and around 97 million reads are obtained after the sequencing

DOI or PMID #

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Summary

The Good

The chip is ready to load the samples unlike the earlier version which need a prolong preparation

The Bad

Costy

The Bottom Line

I will definitely recommend this chip kit if you are working with ion proton /S5 platform for your sequencing.

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