Purification of Immunoglobulin G (IgG) from CHO Cell Culture Expressing Human IgG

June 22, 2020

University of Birmingham
School of Biosciences
Postdoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

GE Healthcare

Product Name:

Protein A-Sepharose® CL-4B

Catalog Number:

17-0780-01

I used GE Healthcare's Protein A Sepharose CL-4B beads for purification and fractionation of recombinant IgG expressed in CHO cell lines. Yield after purification was very good with 15 mg/mL of CHO cell culture. Further purified IgG was used in ELISA assays and gave satisfactory results.

Experimental Design and Results Summary

Application

Immunoprecipitation of immunoglobulin G (IgG) from mammalian cell culture

Starting Material

CHO cell culture expressing recombinant IgG

Tips

None

Results Summary

Using Protein A Sepharose CL-4B beads, nearly 15 to 20 mg of IgG/ mL of CHO cell culture was purified. Further purified IgG was able to antigen and used down stream applications such as ELISAs.

DOI or PMID #

N/A

Related Categories

Image Gallery

Summary

The Good

Efficient purification of immunoglobulins from mammalian cell cultures. Easy to use or pack the beads in the column purification method.

The Bad

None

The Bottom Line

Essential reagent for purification of immunoglobulins form various types of samples. Highly recommended.

Join the discussion