Quantitative PCR for Detection of Changes in the Gene Expression

June 19, 2020

University of Birmingham
School of Biosciences
Postdoctoral Research Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad Laboratories

Product Name:

CFX96 Touch Real Time PCR System

Catalog Number:

1855195

Simultaneous detection of the changes in gene expressions from limited supply samples were performed in a CFX96 touch real time PCR detection system by multiplexing strategy using TaqMan probes. qPCR results were satisfactory with relative quantification using Bio Rad's CFX96 real time PCR system.

Experimental Design and Results Summary

Application

Quantitative detection of changes in the gene expressions in bacterial RNA samples

Tips

Designing probes internal to amplifying primers with minimal secondary structures is critical for success of multiplexed amplification of targets.

DOI or PMID #

N/A

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Summary

The Good

Small foot print. Easy to set up and installation. Setting up assay plates is very intuitive suing CFX96 software. This PCR system is compatible for automation.

The Bad

None

The Bottom Line

Performs well for detection of changes in the gene expression. Easy to use and user friendly. Ideal equipment for molecular diagnostics laboratory.

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