Anti-Rabbit IgG HRP Antibody Is Very Suitable for Western Blotting

June 17, 2020

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Anti-rabbit IgG, HRP-linked Antibody

Catalog Number:

7074

The Anti-rabbit IgG, HRP-linked Antibody functioned well as a secondary antibody for western blotting of protein lysates from human breast cancer cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cells

Primary Incubation

1:1000 overnight at 4 degrees

Blocking Agent

5% BSA

Secondary Incubation

1:2000 for 1 hr at RT

Tertiary Incubation

2 min ECL

Detection

ECL reagents

Results Summary

Very clear specific bands were visualized. The bands for target proteins were thus reliable.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very clear bands

The Bad

N/A

The Bottom Line

There is not much non-specificity with this secondary antibody. It is very comparable with other available rabbit secondary antibodies in the market.

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