BioRad iTaq Universal SYBR for RealTime PCR Gene Expression Studies

Osmania University
Department of Biochemistry
Research Fellow

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Company:

BIO-RAD

Product Name:

iTAQ universal SYBR green supermix

Catalog Number:

1725121

BIORAD iTaq universal SYBR for realtime provides a robust 2x Pmastermix for setting up a real-time PCR reaction. The SYBR 2X mix is very sensitive in detecting a very low copy number cDNA copies

Experimental Design and Results Summary

Application

Quantitative real time PCR

Starting Material

cDNA

Tips

20 microliter reaction is appropriate than 50 microliter reaction

Results Summary

This SYBR master mix was able to detect low copy number of gene .which is not possible with other 2x SYBR master mix

DOI or PMID #

N/A

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Summary

The Good

Very sensitive 2X master mix

The Bad

Nil

The Bottom Line

If you are working with cfx 96./ cfx 384 realtime PCR system I will defiantly recommend this master mix for your PCR analysis

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