Get High-Quality RNA with the Direct-zol RNA MiniPrep Kit

June 04, 2020

UAB
Radiation Oncology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zymo Research

Product Name:

Direct-zol RNA MinipPrep

Catalog Number:

R2050

The kit was used to extract RNA from human breast cancer cells treated with various agents in 2D monolayer culture plates. RT-PCR was subsequently performed with the isolated RNA.

Experimental Design and Results Summary

Application

RT-PCR

Starting Material

Human breast cancer cells

Tips

Make sure to obtain the Tri Reagent to lyse cells as this reagent comes separately!

Results Summary

Nanodrop was used to measure RNA concentration and quality. Considerable amount of RNA was extracted and the quality was comparable to kits from other companies such as Qiagen.

DOI or PMID #

N/A

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Summary

The Good

Easy to use

The Bad

Reagent to lyse cells is sold separately (it should be included in kit!)

The Bottom Line

The kit is great for isolating RNA in a quick and easy manner. The quality of the RNA is sufficient for performance of RT-PCR with reliable results. The kit appears to be more affordable than the more popular ones from companies such as Qiagen.

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