Quality Kit for cDNA Synthesis

National Dairy Research Institute
Animal Biochemistry
Scientist

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Company:

Thermo Fisher Scientific-Invitrogen

Product Name:

SuperScript III First-Strand Synthesis System for RT-PCR

Catalog Number:

18080-051

RNA was extracted from the liver tissue of rats by Trizol reagent. 2 microgram of RNA was used to synthesize cDNA by using the SuperScript III First-Strand Synthesis System for RT-PCR kit. The synthesized cDNA was used to do gene expression studies

Experimental Design and Results Summary

Application

Gene expression studies

Starting Material

Mammalian cells

Tips

N/A

Results Summary

The concentration and purity of cDNA was satisfactory. The prepared cDNA was amplified by PCR and observed in agarose gel.

DOI or PMID #

N/A

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Summary

The Good

Quality of the DNA was good.

The Bad

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The Bottom Line

Excellent kit for the synthesis of cDNA from RNA by SuperScript III First-Strand Synthesis System

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