A Medium Base and Supplement for Dopaminergic Neuron Differentiation

Havard Medical School
Dpt of Cell Bio
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Floor Plate Cell Expansion Kit

Catalog Number:

A3165801

This media is specialized for hPSC differentiation to dopaminergic neurons. The kit includes very explicit protocol which was very helpful.

Experimental Design and Results Summary

Application

DA neuronal differentiation from hESCs

Starting Material

Embryonic Stem Cells

Tips

The supplements should not be left on room temperature longer than 1 hour.

Results Summary

I found this kit and the protocol very helpful. I am the only person working on differentiated dopaminergic neurons as a part of the collaboration, and I got so much help from Thermo manual and tech service.

DOI or PMID #

N/A

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Summary

The Good

Thorough instruction and explanation

The Bad

Expensive

The Bottom Line

Works well, will give ~30% DA neurons (TH+) after differentiation.

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