Fisher Scientific
Hydrogen Peroxide, 30% (Certified ACS)
H325
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Hydrogen peroxide was used to block endogenous peroxidases in the tissue specimens when preparing for immunohistochemistry (immunofluorescence). The diluted solution in PBS (3% final concentration) enabled minimization of false positives when analyzing the samples under the microscope.
Immunocytochemistry (Immunofluorescence)
Mouse breast tumor tissue
Dilute hydrogen peroxide to 3% in PBS for usage
False positives were reduced and staining with antibodies appeared to be quite effective in the tissue specimens.
N/A
Very easy to use
Hydrogen peroxide at 3% concentration should be used to reduce false positives in the specimens and elevate staining of target proteins for IHC.