CST NeuN Antibody Is Ok - Had Some Boundary Effect

May 05, 2020

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

NeuN

Catalog Number:

24307

Clone Number:

Monoclonal

This antibody had some boundary effect and didn't show robust staining.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse DRG

Primary Incubation

1:200

Blocking Agent

5%NDS

Secondary Incubation

1:1000

Tertiary Incubation

None

Detection

Confocal Analysis

Results Summary

This antibody had some staining in the correct cells in murine DRG but should some boundary staining and off target staining in non neuronal cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works ok

The Bad

Not the best for neuron staining

The Bottom Line

This product can work with optimization.

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