Very Sensitive ECL Substrate for Film-Based Western Blot

May 20, 2020

Seoul National University
Biology
Postdoctoral Researcher

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Company:

Bio-Rad

Product Name:

Clarity Max Western ECL Substrate

Catalog Number:

1705062

ECL solution to detect western blot bands using HRP-conjugated secondary antibody. I used film for the development, and it worked very well.

Experimental Design and Results Summary

Application

Western blotting using HRP-conjugated secondary mouse/rabbit antibody

Starting Material

N/A

Tips

You can use 1ml of each for midi membrane. Directly transfer them onto the membrane using a pipette

Results Summary

This is a sensitive ECL solution for western blotting, especially when using an HRP-conjugated secondary antibody. I found the quality of this product consistent.

DOI or PMID #

N/A

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Summary

The Good

Sensitive and consistent quality

The Bad

Nothing

The Bottom Line

This is a good item for western blotting. However, I switched to fluorescent dye conjugated secondary antibodies last year, which gives less sensitivity than HRP (side-by-side comparison), but more consistent band-patterns for most antibodies.

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