Invitrogen by Thermo Fisher Scientific
GeneArt Precision gRNA Synthesis Kit
A29377
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Easy to follow kit to generate gRNA in vitro for CRISPR-knock in and knock out.
gRNA synthesis for CRISPR knock-in and knock-out
N/A
Make sure to clean the bench for RNA synthesis
This kit works always. After the gRNA synthesis, I check the efficiency using nanodrop, and normally the yield is several milligrams.
Easy instruction and high reproducibility
Nothing bad found
This is a great kit to in-house synthesis of gRNA for Cas-9 protein-mediated CRISPR gene engineering.