Best Anti-Rat 555 Secondary Antibody

May 05, 2020

Pharmaceutical and Biomedical Sciences
College of Pharmacy
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594

Catalog Number:

A21209

Clone Number:

N/A

We routinely use this donkey anti rat secondary antibody to detect various primary antibodies at TRITC channel.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:200

Blocking Agent

Blocking Buffer (BSA, Donkey Serum and Triton X100)

Secondary Incubation

1:400

Tertiary Incubation

2 hr

Detection

Immunofluorescence

Results Summary

Gives a quality and bright florescence signal.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good antibody

The Bad

Nothing

The Bottom Line

Recommended

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