Bright, Fixation-Resistant Fluorophore to Stain CD4

April 24, 2020

Surgery
NYU Langone Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 711™ anti-mouse CD4 Antibody

Catalog Number:

100549

Clone Number:

RM4-5

Easy staining procedure and all advantages of polymer dye.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine pancreas with orthotopically injected murine pancreas cancer cells

Primary Incubation

Incubated at 4C for 1h, dilution 1:500

Blocking Agent

1% FACS, anti-CD16/CD32, Mouse serum, Rat IgG

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT

Results Summary

Bright polymer dye with nice population separation with even the lowest laser voltage settings.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High brightness index, stable during fixation

The Bad

Some unexpected bleeding into PerCP/Cy5.5

The Bottom Line

Excellent staining from a Brilliant Violet dye with all its advantages.

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