Detection of CD8 T Cells

April 22, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cyanine5.5 anti-mouse CD8a Antibody

Catalog Number:

100734

Clone Number:

53-6.7

We are interested in detecting CD8 T cells in various tissues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200, 30 minutes, room temperature

Blocking Agent

FC Block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 Yeti Biorad flow cytometer

Results Summary

CD4 and CD8 T cells identified in mouse splenocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Almost no overlap with other colors

The Bad

Not the best separation

The Bottom Line

Okay if this is the only color available for detection of CD8s.

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