Detection of Transferred 2D2 T cells

April 16, 2020

microbiology
NYU
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse TCR Vα3.2 [b, c] Antibody

Catalog Number:

135404

Clone Number:

RR3-16

We are detecting CD45.2 2D2 T cells transferred into CD45.1 hosts.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:200, 20 min, room temperature

Blocking Agent

FC Block

Secondary Incubation

-

Tertiary Incubation

-

Detection

ZE5 YETI Biorad flow cytometer

Results Summary

Transferred TCR V alpha 3.2 T cells were detectable in the spleens of CD45.1 hosts

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use

The Bad

Separation is not that great; Reduced # of colors in panel for reliable detection of cells

The Bottom Line

Okay for just detecting whether cells are transferred or not.

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