Distinct Populations with This TCR V delta 1 Antibody When Used at 1:100

April 13, 2020

Cell Bio
Inhibrx
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

TCR V delta 1 Monoclonal Antibody (TS8.2), PE, eBioscience™

Catalog Number:

12-5679-42

Clone Number:

TS8.2

Here I was trying to distinguish between the Vd1 and Vd2 populations of gamma delta T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs enriched from whole blood

Primary Incubation

30 minutes 4*C

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

This antibody worked as expected. When used at 1:100 there was a distinct Vd1+ population. Interestingly, it looked like there was a Vd1 (hi) and a Vd1(lo) population across multiple donors.

DOI or PMID #

N/A

Additional Notes

Used at 1:100

Related Categories

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Summary

The Good

Worked great at 1:100

The Bad

Nothing bad

The Bottom Line

This antibody did what I wanted it to do.

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