Reliable TNF-Alpha ELISA Kit

Northwestern University
BME
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Mouse TNF-ALPHA ELISA KIT

Catalog Number:

430904

Goal of the project was to optimize the anti-inflammatory treatment for atherosclerosis. I was looking at the mouse serum tnf-alpha levels after treatment with anti-inflammatory drug loaded nanoparticles.

Experimental Design and Results Summary

Application

Analysis of TNF-alpha

Starting Material

Serum, cell culture supernatants

Tips

Usage of freshly reconstituted TNF-alpha standard is preferable for each run.

Results Summary

TNF-alpha levels in the serum and cell culture supernatant were detectable as low as 10 pg/mL.

DOI or PMID #

30601470

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Summary

The Good

Accuracy in detecting Tnf-alpha

The Bad

None

The Bottom Line

High-quality ELISA KIT and highly recommend it for the analysis of both serum and cell culture supernatants.

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