Protein Concentration in Small Volume

March 13, 2020

Harvard Medical School
Cell Biology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Amicon Ultra-0.5 mL Centrifugal Filters for DNA and Protein Purification and Concentration

Catalog Number:

UFC500396

Convenient filter to concentrate bacteria purified proteins in small volume

Experimental Design and Results Summary

Application

Protein purification

Starting Material

N/A

Tips

It takes quite long time to concentration proteins using this

Results Summary

This is a small-sized filter that I use to concentration ubiquitin (8Kda)

DOI or PMID #

N/A

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Summary

The Good

Easy and convenient to use

The Bad

Not compatible with direct mass-spec analysis without a column

The Bottom Line

I always keep this filter on my bench as this is so useful. But sometimes, it takes quite a long time to concentrate.

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